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HDGFL1

ANTIBODY INFORMATION
 
 

Antibody HPA045679

  

Antibody HPA063707

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA045679   HPA063707  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA045679

  

Antibody HPA063707

  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045679

  

Antibody HPA063707

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA063707 across 72 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA045679 across 72 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human testis shows strong nuclear positivity in subset of cells in seminiferus ducts.   Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 1 cell types		   RNA: detected in 1 tissues
Protein: detected in 2 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:3300  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA045679

  

Antibody HPA063707

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 27.2   27.2  

Antibody dilution

 1:250   1:460  

PROTEIN ARRAY
 
 

Antibody HPA045679

  

Antibody HPA063707

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:5550  

ANTIGEN INFORMATION
 
 

Antibody HPA045679

  

Antibody HPA063707

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 28   33  

Antigen sequence

 
ELREEEVADEEASQEWHAEAPGGGDRDS
  
TAVDEESPFLVAVENGSAPSEPGLVCEPPQPEE
 

Matching transcripts

 HDGFL1-001 - ENSP00000442129 [100%]
  HDGFL1-001 - ENSP00000442129 [100%]
 

Other gene match

    
ANTIGEN VIEW
HDGFL1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.