Cell atlas - GMNN - The Human Protein Atlas

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GMNN

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TISSUE

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CELL

CANCER

CELL ATLAS RNA EXPRESSION HUMAN CELLS CUSTOM DATA

GENE/PROTEIN

Antibody validation

Dictionary
	Nucleoplasm

Human cell
	Nucleoplasm

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GENERAL INFORMATION
Gene name	GMNN
Gene description	Geminin, DNA replication inhibitor
Protein class	Cancer-related genes Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	6
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Mainly localized to the nucleoplasm.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (validated)
Single-cell variation	Single-cell variation in protein expression observed.
Cell cycle dependency	Cell cycle dependent expression determined by custom assay

DATA RELIABILITY
Reliability score	Validated based on 3 antibodies. CAB047328 , HPA049977 , HPA054597
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Mainly localized to the nucleoplasm.

Main location

Nucleoplasm (validated)


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail	Antibody	Cell line	Location	Single-cell variation	Cell cycle dependent variation
	CAB047328	A-431	Nucleoplasm
	CAB047328	A-431	Cytosol
	CAB047328	U-2 OS	Nucleoplasm
	CAB047328	U-2 OS	Cytosol
	CAB047328	U-251 MG	Nucleoplasm
	CAB047328	U-251 MG	Cytosol
	HPA049977	A-431	Nucleus	Intensity
	HPA049977	U-2 OS	Nucleus
	HPA049977	U-2 OS	Cytosol
	HPA049977	U-251 MG	Nucleus
	HPA054597	A-431	Nucleus	Intensity
	HPA054597	U-2 OS	Nucleus	Intensity	Intensity
	HPA054597	U-251 MG	Nucleus	Intensity

CUSTOM DATA


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Thumbnail	Antibody	Cell line	Assay	Description
	HPA054597	U-2 OS	Image	Staining of U-2 OS FUCCI cells, to characterize the cell cycle dependency of the protein expression pattern. The FUCCI cells, Fluorescence Ubiquitination Cell Cycle Indicator, are cells tagged with different fluorescent proteins fused to two different cell cycle regulators Cdt1 (expressed in G1 phase) and Geminin (expressed in S and G2 phases) that allows cell cycle monitoring. When both proteins are present, the overlay of the images appear in yellow marking the G1/S transition. GMNN is mostly expressed at the G1/S checkpoint, during S and/or G2.

CELL CYCLE
Summary	Expression has cell cycle dependency according to custom assay

GENE INFORMATION
Gene name	GMNN (HGNC Symbol)
Synonyms	Gem
Description	Geminin, DNA replication inhibitor (HGNC Symbol)
Entrez gene summary	This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]
Chromosome	6
Cytoband	p22.3
Chromosome location (bp)	24774931 - 24786099
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000112312 (version 83.38)
Entrez gene	51053
UniProt	O75496 (UniProt - Evidence at protein level)
neXtProt	NX_O75496
Antibodypedia	GMNN antibodies

PROTEIN BROWSER

GMNN-001	GMNN-002	GMNN-003	GMNN-004	GMNN-006	GMNN-201

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
GMNN-001 ENSP00000230056 ENST00000230056	O75496 [Direct mapping] Geminin A0A024QZY7 [Target identity:100%; Query identity:100%] Geminin, DNA replication inhibitor, isoform CRA_a Show all	Predicted intracellular proteins Cancer-related genes Candidate cancer biomarkers Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000278 [mitotic cell cycle] GO:0003714 [transcription corepressor activity] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0006275 [regulation of DNA replication] GO:0006461 [protein complex assembly] GO:0008156 [negative regulation of DNA replication] GO:0009887 [organ morphogenesis] GO:0042826 [histone deacetylase binding] GO:0045786 [negative regulation of cell cycle] GO:0045892 [negative regulation of transcription, DNA-templated] GO:0070491 [repressing transcription factor binding] Show all	209 aa 23.6 kDa	No	0
GMNN-002 ENSP00000348902 ENST00000356509	O75496 [Direct mapping] Geminin A0A024QZY7 [Target identity:100%; Query identity:100%] Geminin, DNA replication inhibitor, isoform CRA_a Show all	Predicted intracellular proteins Cancer-related genes Candidate cancer biomarkers Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000278 [mitotic cell cycle] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0006275 [regulation of DNA replication] GO:0008156 [negative regulation of DNA replication] GO:0042826 [histone deacetylase binding] GO:0045786 [negative regulation of cell cycle] GO:0045892 [negative regulation of transcription, DNA-templated] Show all	209 aa 23.6 kDa	No	0
GMNN-003 ENSP00000367293 ENST00000378054	E2QRF9 [Direct mapping] Geminin Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005634 [nucleus] GO:0005737 [cytoplasm] GO:0006275 [regulation of DNA replication] GO:0008156 [negative regulation of DNA replication] GO:0045786 [negative regulation of cell cycle] Show all	179 aa 20.5 kDa	No	0
GMNN-004 ENSP00000419584 ENST00000476555	C9K0U5 [Direct mapping] Geminin Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0006275 [regulation of DNA replication] GO:0008156 [negative regulation of DNA replication] GO:0045786 [negative regulation of cell cycle] Show all	66 aa 7.3 kDa	No	0
GMNN-006 ENSP00000367298 ENST00000378059	H7C608 [Direct mapping] Geminin Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005634 [nucleus] GO:0006275 [regulation of DNA replication] GO:0008156 [negative regulation of DNA replication] GO:0045786 [negative regulation of cell cycle] Show all	102 aa 11.4 kDa	No	0
GMNN-201 ENSP00000477506 ENST00000620958	O75496 [Direct mapping] Geminin A0A024QZY7 [Target identity:100%; Query identity:100%] Geminin, DNA replication inhibitor, isoform CRA_a Show all	Predicted intracellular proteins Cancer-related genes Candidate cancer biomarkers Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000278 [mitotic cell cycle] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0005829 [cytosol] GO:0006275 [regulation of DNA replication] GO:0008156 [negative regulation of DNA replication] GO:0042826 [histone deacetylase binding] GO:0045786 [negative regulation of cell cycle] GO:0045892 [negative regulation of transcription, DNA-templated] Show all	209 aa 23.6 kDa	No	0

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.