Fields »
ABOUT
HELP
BLOG
EPM2A

ANTIBODY INFORMATION
 
 

Antibody HPA053643

  

Antibody HPA055468

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA053643   HPA055468  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA053643

  

Antibody HPA055468

  
Immunocytochemistry


Validated
  

Validated
  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA053643

  

Antibody HPA055468

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA055468.		  
Validated

Antibody staining overlaps with antibody HPA053643.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.  

Antibody dilution

 1:33   1:5  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA053643

  

Antibody HPA055468

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 37.2, 35.5, 27.1, 22.2, 19.5   37.2, 22.2  

Antibody dilution

 1:160   1:80  

PROTEIN ARRAY
 
 

Antibody HPA053643

  

Antibody HPA055468

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1300   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA053643

  

Antibody HPA055468

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 115   78  

Antigen sequence

 
ATGHTNEMKHTTDFYFNIAGHQAMHYSRILPNIWLGSCPRQVEHVTIKLK
HELGITAVMNFQTEWDIVQNSSGCNRYPEPMTPDTMIKLYREEGLAYIWM
PTPDMSTEGRVQMLP
  
HALLEKGHIVYVHCNAGVGRSTAAVCGWLQYVMGWNLRKVQYFLMAKRPA
VYIDEEALARAQEDFFQKFGKVRSSVCS
 

Matching transcripts

 EPM2A-002 - ENSP00000356489 [100%]
EPM2A-004 - ENSP00000405913 [100%]
EPM2A-202 - ENSP00000480339 [100%]
EPM2A-001 - ENSP00000414900 [94%]
EPM2A-201 - ENSP00000480268 [94%]
  EPM2A-002 - ENSP00000356489 [100%]
EPM2A-201 - ENSP00000480268 [100%]
 

Other gene match

    
ANTIGEN VIEW
EPM2A-001
EPM2A-002
EPM2A-004
EPM2A-201
EPM2A-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.