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Immunohistochemical staining of retina shows strong cytoplasmic positivity in photoreceptors.
Expression
RNA: detected in 16 tissues Protein: detected in 0 cell types
Retrieval
HIER pH6
Antibody dilution
1:2000
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA029458
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400122)
Target mass (kDa)
39.3
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA029458
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.