Fields »
ABOUT
HELP
BLOG
BTNL8

ANTIBODY INFORMATION
 
 

Antibody HPA039738

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039738  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 10  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA039738

  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039738

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 14 tissues
Protein: detected in 42 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:100  

Literature conformity

 Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039738

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 56.7, 43.6, 42.9, 36.2, 33.8  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA039738

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA039738

  

Antigen

 Recombinant protein fragment  

Length (aa)

 52  

Antigen sequence

 
LLRPYIEYPSYNEQNGTPIVICPVTQESEKEASWQRASAIPETSNSESSS
QA
 

Matching transcripts

 BTNL8-002 - ENSP00000342197 [100%]
BTNL8-003 - ENSP00000383543 [100%]
BTNL8-005 - ENSP00000425207 [100%]
BTNL8-006 - ENSP00000427441 [100%]
BTNL8-201 - ENSP00000435098 [100%]
BTNL8-202 - ENSP00000483717 [100%]
 

Other gene match

  
ANTIGEN VIEW
BTNL8-002
BTNL8-003
BTNL8-005
BTNL8-006
BTNL8-201
BTNL8-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.