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RPL24

ANTIBODY INFORMATION
 
 

Antibody HPA051653

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA051653  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 12  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA051653

  
Immunocytochemistry


Validated
  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051653

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		  

 

Relative fluorescence intensity (RFI)

  

Figure description

 Cytoplasmic region of segmented cells in 10x-images  

Antibody dilution

 1:9  
 

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to cytosol & endoplasmic reticulum.  

Antibody dilution

 1:9  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051653

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 70 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:50  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051653

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 17.8, 17.5, 14.4  

Antibody dilution

 1:90  

PROTEIN ARRAY
 
 

Antibody HPA051653

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:700  

ANTIGEN INFORMATION
 
 

Antibody HPA051653

  

Antigen

 Recombinant protein fragment  

Length (aa)

 51  

Antigen sequence

 
FLSKRNPRQINWTVLYRRKHKKGQSEEIQKKRTRRAVKFQRAITGASLAD
I
 

Matching transcripts

 RPL24-001 - ENSP00000377640 [100%]
RPL24-003 - ENSP00000419179 [100%]
RPL24-007 - ENSP00000420380 [100%]
 

Other gene match

  
ANTIGEN VIEW
RPL24-001
RPL24-003
RPL24-007
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.