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ABTB1

ANTIBODY INFORMATION
 
 

Antibody HPA035022

  

Antibody HPA035023

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA035022   HPA035023  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   6  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA035022

  

Antibody HPA035023

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035022

  

Antibody HPA035023

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.  

Antibody dilution

 1:200   1:128  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035022

  

Antibody HPA035023

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 61 cell types		   RNA: detected in 37 tissues
Protein: detected in 58 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:700  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035022

  

Antibody HPA035023

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 54, 37.6   54, 37.6  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA035022

  

Antibody HPA035023

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:12000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA035022

  

Antibody HPA035023

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 99   102  

Antigen sequence

 
GVEHVSDCERLAKQCQLWDLLSDLEAKCEKVSEFVASKPGTCVKVLTIEP
PPADPRLREDMALLADCALPPELRGDLWELPFPCPDGFNSCPDICFRVA
  
MLDEDTVVGVWRVAKLFRLARLEDQCTEYMAKVIEKLVEREDFVEAVKEE
AAAVAARQETDSIPLVDDIRFHVASTVQTYSAIEEAQQRLRALEDLLVSI
GL
 

Matching transcripts

 ABTB1-001 - ENSP00000232744 [100%]
ABTB1-004 - ENSP00000412684 [100%]
ABTB1-006 - ENSP00000417366 [100%]
  ABTB1-001 - ENSP00000232744 [100%]
ABTB1-004 - ENSP00000412684 [100%]
ABTB1-006 - ENSP00000417366 [100%]
 

Other gene match

    
ANTIGEN VIEW
ABTB1-001
ABTB1-004
ABTB1-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.