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LMAN2L

ANTIBODY INFORMATION
 
 

Antibody HPA026600

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA026600  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 5  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA026600

  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA026600

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:40  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA026600

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 41, 39.7  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA026600

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA026600

  

Antigen

 Recombinant protein fragment  

Length (aa)

 138  

Antigen sequence

 
RVFPYISAMVNNGSLSYDHERDGRPTELGGCTAIVRNLHYDTFLVIRYVK
RHLTIMMDIDGKHEWRDCIEVPGVRLPRGYYFGTSSITGDLSDNHDVISL
KLFELTVERTPEEEKLHRDVFLPSVDNMKLPEMTAPLP
 

Matching transcripts

 LMAN2L-001 - ENSP00000264963 [100%]
LMAN2L-007 - ENSP00000366280 [100%]
 

Other gene match

  
ANTIGEN VIEW
LMAN2L-001
LMAN2L-007
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.