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FAHD2A

ANTIBODY INFORMATION
 
 

Antibody HPA042145

  

Antibody HPA044987

  

Antibody CAB045971

  

Antibody CAB045972

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Origene
   Origene
  

Product name

 HPA042145   HPA044987   TA500944   TA500993  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

 FAHD2B - ENSG00000144199 [100%]
   FAHD2B - ENSG00000144199 [100%]
      

Released in version

 9   9   12   9  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA042145

  

Antibody HPA044987

  

Antibody CAB045971

  

Antibody CAB045972

  
Immunohistochemistry

Supported   Supported   Supported   Supported  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Supported   Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042145

  

Antibody HPA044987

  

Antibody CAB045971

  

Antibody CAB045972

  

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 53 cell types		   RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 63 cell types		   RNA: detected in 37 tissues
Protein: detected in 49 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:300   1:600   1:1300  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042145

  

Antibody HPA044987

  

Antibody CAB045971

  

Antibody CAB045972

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 34.6   34.6, 14, 9   34.6, 14, 9   34.6, 14, 9  

Antibody dilution

 1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA042145

  

Antibody HPA044987

  

Antibody CAB045971

  

Antibody CAB045972

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:6000      

ANTIGEN INFORMATION
 
 

Antibody HPA042145

  

Antibody HPA044987

  

Antibody CAB045971

  

Antibody CAB045972

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 83   75      

Antigen sequence

 
NYVDHCKEQNVPVPKEPIIFSKFASSIVGPYDEVVLPPQSQEVDWEVELA
VVIGKKGKHIKATDAMAHVAGFTVAHDVSARDW
  
KWPFQPSRDMRLVQFRAPHLVGPHLGLETGNGGGVINLNAFDPTLPKTMT
QFLEQGEATLSVARRALAAQLPVLP
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					FAHD2A-001 - ENSP00000233379 [100%] 
FAHD2A-002 - ENSP00000406424 [100%] 

											 
																						FAHD2A-001 - ENSP00000233379 [100%] 
FAHD2A-002 - ENSP00000406424 [100%] 
FAHD2A-006 - ENSP00000390528 [100%] 
FAHD2A-005 - ENSP00000405148 [85%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					FAHD2B - ENSG00000144199 [100%]

											 
																						FAHD2B - ENSG00000144199 [100%]

											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
FAHD2A-001
FAHD2A-002
FAHD2A-005
FAHD2A-006
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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