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Antibody staining overlaps with antibody HPA037564.
Validated
Antibody staining overlaps with antibody HPA037563.
Standard validation
Approved
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
Antibody dilution
1:79
1:122
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA037563
Antibody HPA037564
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human kidney shows distinct membranous positivity in cells in glomeruli.
Expression
RNA: detected in 36 tissues Protein: detected in 17 cell types
RNA: detected in 36 tissues Protein: detected in 21 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:300
1:450
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA037563
Antibody HPA037564
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412211)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412211)
Target mass (kDa)
81.9, 81.7
81.9, 81.7, 77.5
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA037563
Antibody HPA037564
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
