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Immunohistochemical staining of human pituitary gland shows strong cytoplasmic positivity in a subset of cells in anterior lobe.
Immunohistochemical staining of human pituitary gland shows strong cytoplasmic positivity in cells in anterior lobe.
Immunohistochemical staining of human pituitary gland shows cytoplasmic positivity in subsets of cells in anterior pituitary gland.
Expression
RNA: detected in 11 tissues Protein: detected in 5 cell types
RNA: detected in 11 tissues Protein: detected in 0 cell types
RNA: detected in 11 tissues Protein: detected in 1 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:2000
1:12000
1:30000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA046135
Antibody HPA063644
Antibody CAB002762
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
29.4
29.4, 27.1
29.4, 27.1
Antibody dilution
1:80
1:310
1:500
PROTEIN ARRAY
Antibody HPA046135
Antibody HPA063644
Antibody CAB002762
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
