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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:15
1:40
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA001237
Antibody HPA005823
Antibody HPA029560
Antibody CAB007779
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic and membranous positivity in cells in tubules.
Immunohistochemical staining of human small intestine shows strong luminal membranous positivity in glandular cells.
Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in glomeruli.
Expression
RNA: detected in 37 tissues Protein: detected in 51 cell types
RNA: detected in 37 tissues Protein: detected in 23 cell types
RNA: detected in 37 tissues Protein: detected in 54 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:600
1:75
1:15
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001237
Antibody HPA005823
Antibody HPA029560
Antibody CAB007779
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
65.4, 62, 51.3, 21, 19.9
65.4, 62, 51.3, 34.4
65.4, 51.3, 34.4
65.4, 62, 51.3, 34.4, 21, 19.9, 13.1, 12.3, 9.2
Antibody dilution
1:250
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA001237
Antibody HPA005823
Antibody HPA029560
Antibody CAB007779
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
VAEKHRGNYTCHASYTYLGKQYPITRVIEFITLEENKPTRPVIVSPANET
MEVDLGSQIQLICNVTGQLSDIAYWKWNGSVIDEDDPVLGEDYYSVENPA
NKRRSTLITVLNISEIESRFYKHPFTCFAKNTHGIDAAYIQLIYPVTNFQ
K
