ATF2 - Antibodies - The Human Protein Atlas

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ATF2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA022134

Antibody CAB003769

Provider

Atlas Antibodies
Sigma-Aldrich

Epitomics

Product name

HPA022134

1489-1

Host species

Rabbit

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Supernatant

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA022134

Antibody CAB003769

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Supported

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA022134

Antibody CAB003769

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Antibody dilution

1:50

1:37

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA022134

Antibody CAB003769

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells.

Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells, cells in molecular layer

Expression

RNA: detected in 37 tissues
Protein: detected in 35 cell types

RNA: detected in 37 tissues
Protein: detected in 71 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:150

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA022134

Antibody CAB003769

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

54.5, 52.3, 48, 42.3, 35

54.5, 52.3, 48, 42.3, 35, 30, 23, 20.3, 15.4

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA022134

Antibody CAB003769

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:750

ANTIGEN INFORMATION

Antibody HPA022134

Antibody CAB003769

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

143

Antigen sequence

SSNVHVPAAVPLVRPVTMVPSVPGIPGPSSPQPVQSEAKMRLKAALTQQH
PPVTNGDTVKGHGSGLVRTQSEESRPQSLQQPATSTTETPASPAHTTPQT
QSTSGRRRRAANEDPDEKRRKFLERNRAAASRCRQKRKVWVQS

Matching transcripts

ATF2-001 - ENSP00000264110 [100%]
ATF2-002 - ENSP00000376327 [100%]
ATF2-012 - ENSP00000387093 [100%]
ATF2-013 - ENSP00000340576 [100%]
ATF2-201 - ENSP00000407911 [100%]
ATF2-202 - ENSP00000437952 [100%]
ATF2-011 - ENSP00000386326 [92%]

Other gene match

ANTIGEN VIEW

ATF2-001	ATF2-002	ATF2-011	ATF2-012	ATF2-013	ATF2-201	ATF2-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.