C2orf42 - Antibodies - The Human Protein Atlas

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C2orf42

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA031840

Antibody HPA031841

Antibody HPA031842

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA031840

HPA031841

HPA031842

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA031840

Antibody HPA031841

Antibody HPA031842

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA031840

Antibody HPA031841

Antibody HPA031842

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA031841.

Validated

Antibody staining overlaps with antibody HPA031840.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A549 shows localization to nucleus.

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.

Antibody dilution

1:5

1:103

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA031840

Antibody HPA031841

Antibody HPA031842

Standard validation

Supported

Figure description

Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.

Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 17 cell types

RNA: detected in 37 tissues
Protein: detected in 23 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:35

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA031840

Antibody HPA031841

Antibody HPA031842

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

64.1

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA031840

Antibody HPA031841

Antibody HPA031842

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3000

ANTIGEN INFORMATION

Antibody HPA031840

Antibody HPA031841

Antibody HPA031842

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

IHQTMHYQFDGKPEPLVFHIPQSFFDALQQRISIGSAKKRLPNSTTAFVR
KDALPLGTFSKYTWHITNILQVKQILDTPEMPLEITRSFIQNRDGTY

KVEVESIAETYGRIEKQPVLRPLELKTFLKVGNTSPDQKEPTPFIIEWIP
DILPQSKIGELRIKFEYGHHRNGHVAEYQDQRPPLDQPLELAPLT

HKSNASKDETAQRCIHFFACICAFASDETLAQEFSDFLNFDSSGLKEIIV
PQLGCHSESTVSACESTASKSKKRRKDEVSG

Matching transcripts

C2orf42-001 - ENSP00000264434 [100%]
C2orf42-002 - ENSP00000404515 [100%]

Other gene match

ANTIGEN VIEW

C2orf42-001	C2orf42-002

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.