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Immunofluorescent staining of human cell line SiHa shows localization to nucleus & cytosol.
Antibody dilution
1:63
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA059153
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human oral mucosa shows moderate nuclear positivity in superficial layers of squamous epithelium.
Expression
RNA: detected in 36 tissues Protein: detected in 23 cell types
Retrieval
HIER pH6
Antibody dilution
1:1600
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA059153
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
43.9
Antibody dilution
1:210
PROTEIN ARRAY
Antibody HPA059153
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
