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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Antibody dilution
1:100
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA027189
Antibody HPA027275
Antibody HPA027281
Antibody HPA027312
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA027281 across 46 cell lines. Pearson correlation with HPA027275 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA027281 across 46 cell lines. Pearson correlation with HPA027189 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA027275 across 46 cell lines. Pearson correlation with HPA027189 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA027281 across 71 cell types. Spearman correlation with HPA027275 across 71 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA027189 across 71 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA027189 across 71 cell types.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human fallopian tube shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human bronchus shows strong membranous positivity in respiratory epithelial cells.
Immunohistochemical staining of human fallopian tube shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human nasopharynx shows strong membranous positivity in respiratory epithelial cells.
Expression
RNA: detected in 18 tissues Protein: detected in 12 cell types
RNA: detected in 18 tissues Protein: detected in 6 cell types
RNA: detected in 18 tissues Protein: detected in 14 cell types
RNA: detected in 18 tissues Protein: detected in 19 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:250
1:400
1:900
1:600
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027189
Antibody HPA027275
Antibody HPA027281
Antibody HPA027312
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
60.1, 60, 48.5, 40.7
60.1, 60, 48.5, 40.7
60.1, 60, 48.5, 40.7
60.1, 60, 48.5
Antibody dilution
1:250
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA027189
Antibody HPA027275
Antibody HPA027281
Antibody HPA027312
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
