We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Antibody staining overlaps with antibody HPA027452. Antibody staining overlaps with antibody HPA027417.
Validated
Antibody staining overlaps with antibody HPA027452. Antibody staining overlaps with antibody HPA027406.
Validated
Antibody staining overlaps with antibody HPA027417. Antibody staining overlaps with antibody HPA027406.
Standard validation
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
Antibody dilution
1:50
1:50
1:50
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA027406
Antibody HPA027417
Antibody HPA027452
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA027452 across 46 cell lines. Pearson correlation with HPA027417 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA027406 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA027406 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA027417 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA027406 across 74 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 79 cell types
RNA: detected in 37 tissues Protein: detected in 79 cell types
RNA: detected in 37 tissues Protein: detected in 78 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:120
1:80
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027406
Antibody HPA027417
Antibody HPA027452
Formal validation: Genetic
Validated
Downregulation visible in both siRNA lanes
Figure description
Lane 1: Marker [kDa] 250, 150, 100, 75, 50, 37, 25, 20, 15, 10 Lane 2: siRNA 1 Lane 3: siRNA 2 Lane 4: Scrambled
