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Antibody staining overlaps with antibody HPA041734.
Validated
Antibody staining overlaps with antibody HPA041664.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleus, nucleoli fibrillar center & cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli fibrillar center & mitochondria.
Antibody dilution
1:200
1:200
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA041664
Antibody HPA041734
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA041734 across 41 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA041664 across 41 cell lines.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human vagina shows moderate nucleolar positivity in squamous epithelial cells.
Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 35 tissues Protein: detected in 52 cell types
RNA: detected in 35 tissues Protein: detected in 54 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:1600
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041664
Antibody HPA041734
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415980)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415980)
Target mass (kDa)
55.2, 55
55.2, 55
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA041664
Antibody HPA041734
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
