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Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 52 cell types
RNA: detected in 36 tissues Protein: detected in 76 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:35
1:125
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA058284
Antibody CAB020701
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
44.9, 40.5, 40.1
44.9, 40.5, 40.1, 8.4
Antibody dilution
1:80
1:500
PROTEIN ARRAY
Antibody HPA058284
Antibody CAB020701
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
