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ARG1

ANTIBODY INFORMATION
 
 

Antibody HPA003595

  

Antibody HPA024006

  

Antibody CAB009434

  

Antibody CAB056159

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA003595   HPA024006   sc-20150   AMAb90545  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 3   5   4   10  

References

 6   3      

VALIDATION SUMMARY
 
 

Antibody HPA003595

  

Antibody HPA024006

  

Antibody CAB009434

  

Antibody CAB056159

  
Immunohistochemistry


Validated
   Supported   Supported  

Validated
  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Supported   Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003595

  

Antibody HPA024006

  

Antibody CAB009434

  

Antibody CAB056159

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		      
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

 

      
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.   Immunohistochemical staining of human liver shows distinct cytoplasmic and nuclear positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.  

Expression

 RNA: detected in 8 tissues
Protein: detected in 5 cell types		   RNA: detected in 8 tissues
Protein: detected in 7 cell types		   RNA: detected in 8 tissues
Protein: detected in 39 cell types		   RNA: detected in 8 tissues
Protein: detected in 4 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:2000   1:3000   1:125   1:4000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003595

  

Antibody HPA024006

  

Antibody CAB009434

  

Antibody CAB056159

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 35.7, 34.7   35.7, 34.7   35.7, 34.7   35.7, 34.7  

Antibody dilution

 1:250   1:250   1:500   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA003595

  

Antibody HPA024006

  

Antibody CAB009434

  

Antibody CAB056159

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA003595

  

Antibody HPA024006

  

Antibody CAB009434

  

Antibody CAB056159

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 119   124      

Antigen sequence

 
TIGIIGAPFSKGQPRGGVEEGPTVLRKAGLLEKLKEQECDVKDYGDLPFA
DIPNDSPFQIVKNPRSVGKASEQLAGKVAEVKKNGRISLVLGGDHSLAIG
SISGHARVHPDLGVIWVDA
  
GLRDVDPGEHYILKTLGIKYFSMTEVDRLGIGKVMEETLSYLLGRKKRPI
HLSFDVDGLDPSFTPATGTPVVGGLTYREGLYITEEIYKTGLLSGLDIME
VNPSLGKTPEEVTRTVNTAVAITL
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					ARG1-001 - ENSP00000357066 [100%] 
ARG1-004 - ENSP00000349446 [94%] 

											 
																						ARG1-001 - ENSP00000357066 [100%] 
ARG1-004 - ENSP00000349446 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ARG1-001
ARG1-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA003595

												
										 
																				
											
											
											
Antibody HPA024006

												
										 
																				
											
											
											
Antibody CAB009434

												
										 
																				
											
											
											
Antibody CAB056159

												
										 
																		

																	

										
											
1.

												
																						A quantitative proteomic approach for identification of potential biomarkers in hepatocellular carcinoma

	Chaerkady R et al

	J Proteome Res 2008;7(10):4289-98

			
											 
																							Expression patterns of the immunomodulatory enzyme arginase 1 in blood, lymph nodes and tumor tissue of early-stage breast cancer patients

	de Boniface J et al

	Oncoimmunology 2012;1(8):1305-1312

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Arginase-1: a new immunohistochemical marker of hepatocytes and hepatocellular neoplasms

	Yan BC et al

	Am J Surg Pathol 2010;34(8):1147-54

			
											 
																							Initial quantitative proteomic map of 28 mouse tissues using the SILAC mouse

	Geiger T et al

	Mol Cell Proteomics 2013;12(6):1709-22

			
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						Local arginase inhibition during early reperfusion mediates cardioprotection via increased nitric oxide production

	Gonon AT et al

	PLoS One 2012;7(7):e42038

			
											 
																							Interleukin-6/interleukin-21 signaling axis is critical in the pathogenesis of pulmonary arterial hypertension

	Hashimoto-Kataoka T et al

	Proc Natl Acad Sci U S A 2015;112(20):E2677-86

			
											 
																						
											 
																						
											 
																				

																		

										
											
4.

												
																						Arginase inhibition restores in vivo coronary microvascular function in type 2 diabetic rats

	Grönros J et al

	Am J Physiol Heart Circ Physiol 2011;300(4):H1174-81

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
5.

												
																						Arginase regulates red blood cell nitric oxide synthase and export of cardioprotective nitric oxide bioactivity

	Yang J et al

	Proc Natl Acad Sci U S A 2013;110(37):15049-54

			
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
6.

												
																						Arginase inhibition improves endothelial function in patients with coronary artery disease and type 2 diabetes mellitus

	Shemyakin A et al

	Circulation 2012;126(25):2943-50

			
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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