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VAMP8

ANTIBODY INFORMATION
 
 

Antibody HPA006882

  

Antibody CAB037132

  

Antibody CAB037147

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SIGMA
   SIGMA
  

Product name

 HPA006882   V7389   V7514  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   msAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity   Affinity  

Other gene match

      

Released in version

 4   13   13  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA006882

  

Antibody CAB037132

  

Antibody CAB037147

  
Immunohistochemistry


Validated
  

Validated
  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Approved      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006882

  

Antibody CAB037132

  

Antibody CAB037147

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

   
 

 

  

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human placenta shows cytoplasmic positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows cytoplasmic and membranous positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows cytoplasmic and membranous positivity in trophoblastic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 44 cell types		   RNA: detected in 37 tissues
Protein: detected in 56 cell types		   RNA: detected in 37 tissues
Protein: detected in 57 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:3000   1:4000  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006882

  

Antibody CAB037132

  

Antibody CAB037147

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 11.4   11.4, 11.2, 8.6   11.4, 11.2, 8.6  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA006882

  

Antibody CAB037132

  

Antibody CAB037147

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA006882

  

Antibody CAB037132

  

Antibody CAB037147

  

Antigen

 Recombinant protein fragment   Synthetic peptide   Synthetic peptide  

Length (aa)

 77      

Antigen sequence

 
MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTED
LEATSEHFKTTSQKVARKFWWKNVKMI
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					VAMP8-001 - ENSP00000263864 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
VAMP8-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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