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RPN2

ANTIBODY INFORMATION
 
 

Antibody HPA008297

  

Antibody HPA025922

  

Antibody CAB019277

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA008297   HPA025922   2750.00.02  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 4   5   5  

References

 1      

VALIDATION SUMMARY
 
 

Antibody HPA008297

  

Antibody HPA025922

  

Antibody CAB019277

  
Immunocytochemistry

  Approved    
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008297

  

Antibody HPA025922

  

Antibody CAB019277

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.    

Antibody dilution

   1:20    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008297

  

Antibody HPA025922

  

Antibody CAB019277

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA025922 across 72 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA008297 across 72 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 69 cell types		   RNA: detected in 37 tissues
Protein: detected in 63 cell types		   RNA: detected in 37 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:50   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA008297

  

Antibody HPA025922

  

Antibody CAB019277

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 69.3, 67.7, 37.2   69.3, 67.7   69.3, 67.7, 37.2, 18.3, 16.8, 15.5  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008297

  

Antibody HPA025922

  

Antibody CAB019277

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:12000    

ANTIGEN INFORMATION
 
 

Antibody HPA008297

  

Antibody HPA025922

  

Antibody CAB019277

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Genetic immunization  

Length (aa)

 123   132    

Antigen sequence

 
SISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALSALTARL
SKEETVLATVQALQTASHLSQQADLRSIVEEIEDLVARLDELGGVYLQFE
EGLETTALFVAATYKLMDHVGTE
  
SSGYYDFLVEVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAP
KTTRVTYPAKAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQ
KTGQEVVFVAEPDNKNVYKFELDTSERKIEFD
   

Matching transcripts

 RPN2-001 - ENSP00000237530 [100%]
RPN2-003 - ENSP00000362724 [100%]
RPN2-002 - ENSP00000362735 [88%]
  RPN2-001 - ENSP00000237530 [100%]
RPN2-003 - ENSP00000362724 [100%]
   

Other gene match

      
ANTIGEN VIEW
RPN2-001
RPN2-002
RPN2-003

RELEVANT PUBLICATIONS
 
 

Antibody HPA008297

  

Antibody HPA025922

  

Antibody CAB019277

  

1.

 Candidate serological biomarkers for cancer identified from the secretomes of 23 cancer cell lines and the human protein atlas
Wu CC et al
Mol Cell Proteomics 2010;9(6):1100-17
      
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.