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Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA024099 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA030387 across 73 cell types. Spearman correlation with HPA019352 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA024099 across 73 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human pancreas shows strong positivity in islet cells.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in tubular cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 21 tissues Protein: detected in 50 cell types
RNA: detected in 21 tissues Protein: detected in 19 cell types
RNA: detected in 21 tissues Protein: detected in 14 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:3000
1:35
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019352
Antibody HPA024099
Antibody HPA030387
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418083)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418083)
Target mass (kDa)
52.8, 51, 44.2, 42.4, 22.2
52.8, 51, 44.2, 42.4, 19.3
52.8, 51
Antibody dilution
1:250
1:250
1:125
PROTEIN ARRAY
Antibody HPA019352
Antibody HPA024099
Antibody HPA030387
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
