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BBOF1

ANTIBODY INFORMATION
 
 

Antibody HPA003090

  

Antibody HPA003136

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA003090   HPA003136  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   2  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA003090

  

Antibody HPA003136

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003090

  

Antibody HPA003136

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nucleus & microtubule organizing center.  

Antibody dilution

   1:154  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003090

  

Antibody HPA003136

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine pancreas and islet cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 56 cell types		   RNA: detected in 35 tissues
Protein: detected in 72 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:150  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA003090

  

Antibody HPA003136

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 62   62  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA003090

  

Antibody HPA003136

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA003090

  

Antibody HPA003136

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 100   147  

Antigen sequence

 
PGEAKMPSKGKDKKKGKSKGKDTKKLIKTDESVVDRAKANASLWEARLEV
TELSRIKYRDTSRILAKSNEDLKKKQCKMEKDIMSVLSYLKKQDQEKDNM
  
ISRKHYKQIAQAAFNLKMRAACTGRTEYPKIRTFDGREHSTNSVNQDLLE
AEKWTHIEGNVDIGDLTWEQKEKVLRLLFAKMNGCPSRKYNQSSRPPVPD
YVVSDSGETKEFGDESKLQDKIFITQQIAISDSSGEVVLPTIPKEPQ
 

Matching transcripts

 BBOF1-001 - ENSP00000377577 [95%]
  BBOF1-001 - ENSP00000377577 [100%]
 

Other gene match

    
ANTIGEN VIEW
BBOF1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.