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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA029075 across 46 cell lines. Pearson correlation with HPA029074 across 45 cell lines. Pearson correlation with HPA029073 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA029075 across 46 cell lines. Pearson correlation with HPA029074 across 45 cell lines. Pearson correlation with HPA029072 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA029075 across 45 cell lines. Pearson correlation with HPA029073 across 45 cell lines. Pearson correlation with HPA029072 across 45 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA029074 across 45 cell lines. Pearson correlation with HPA029073 across 46 cell lines. Pearson correlation with HPA029072 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA029075 across 74 cell types. Spearman correlation with HPA029074 across 74 cell types. Spearman correlation with HPA029073 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA029075 across 74 cell types. Spearman correlation with HPA029074 across 74 cell types. Spearman correlation with HPA029072 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA029075 across 75 cell types. Spearman correlation with HPA029073 across 74 cell types. Spearman correlation with HPA029072 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA029074 across 75 cell types. Spearman correlation with HPA029073 across 74 cell types. Spearman correlation with HPA029072 across 74 cell types.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human liver shows strong cytoplasmic positivity with a granular pattern in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity(granular pattern) in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity with a granular pattern in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
RNA: detected in 37 tissues Protein: detected in 43 cell types
RNA: detected in 37 tissues Protein: detected in 49 cell types
RNA: detected in 37 tissues Protein: detected in 47 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:400
1:600
1:800
1:550
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029072
Antibody HPA029073
Antibody HPA029074
Antibody HPA029075
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
57.8, 56.2
57.8, 56.2, 27
57.8, 56.2
57.8, 56.2, 27
Antibody dilution
1:250
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA029072
Antibody HPA029073
Antibody HPA029074
Antibody HPA029075
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
