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EIF2B2

ANTIBODY INFORMATION
 
 

Antibody HPA005841

  

Antibody HPA048028

  

Antibody CAB037105

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA005841   HPA048028   E6282  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 3   12   13  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA005841

  

Antibody HPA048028

  

Antibody CAB037105

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA005841

  

Antibody HPA048028

  

Antibody CAB037105

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, plasma membrane & focal adhesion sites.    

Antibody dilution

   1:154    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA005841

  

Antibody HPA048028

  

Antibody CAB037105

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in chief cells.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 70 cell types		   RNA: detected in 36 tissues
Protein: detected in 71 cell types		   RNA: detected in 36 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:400   1:500  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA005841

  

Antibody HPA048028

  

Antibody CAB037105

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 39   39   39, 7.4  

Antibody dilution

 1:250   1:770   1:500  

PROTEIN ARRAY
 
 

Antibody HPA005841

  

Antibody HPA048028

  

Antibody CAB037105

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000   1:6150    

ANTIGEN INFORMATION
 
 

Antibody HPA005841

  

Antibody HPA048028

  

Antibody CAB037105

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 150   151    

Antigen sequence

 
PFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGAL
RAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVL
PFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYH
  
RETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRV
LKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIE
AINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKR
K
  

											 
																				

																		

										
											
Matching transcripts

												
																					EIF2B2-001 - ENSP00000266126 [100%] 

											 
																						EIF2B2-001 - ENSP00000266126 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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