Fields »
ABOUT
HELP
BLOG
KLHL29

ANTIBODY INFORMATION
 
 

Antibody HPA049057

  

Antibody HPA057379

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA049057   HPA057379  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA049057

  

Antibody HPA057379

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved    
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA049057

  

Antibody HPA057379

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol & mitochondria.  

Antibody dilution

   1:36  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049057

  

Antibody HPA057379

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells of seminiferus ducts.    

Expression

 RNA: detected in 28 tissues
Protein: detected in 45 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:75    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA049057

  

Antibody HPA057379

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 94.2, 59.1   94.2, 59.1  

Antibody dilution

 1:80   1:120  

PROTEIN ARRAY
 
 

Antibody HPA049057

  

Antibody HPA057379

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:650   1:1300  

ANTIGEN INFORMATION
 
 

Antibody HPA049057

  

Antibody HPA057379

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 104   89  

Antigen sequence

 
SNCLGVLAMAEAMQCSELYHMAKAFALQIFPEVAAQEEILSISKDDFIAY
VSNDSLNTKAEELVYETVIKWIKKDPATRTQYAAELLAVVRLPFIHPSYL
LNVV
  
GVAEVIVLVGGRQMVGMTQRSLVAVTCWNPQNNKWYPLASLPFYDREFFS
VVSAGDNIYLSGGMESGVTLADVWCYMSLLDNWNLVSRM
 

Matching transcripts

 KLHL29-001 - ENSP00000420659 [100%]
KLHL29-003 - ENSP00000288548 [100%]
  KLHL29-001 - ENSP00000420659 [100%]
KLHL29-003 - ENSP00000288548 [100%]
 

Other gene match

    
ANTIGEN VIEW
KLHL29-001
KLHL29-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.