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Immunohistochemical staining of human kidney shows moderate membranous positivity in cells in tubules.
Immunohistochemical staining of human vulva/anal skin shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in bile duct cells.
Expression
RNA: detected in 7 tissues Protein: detected in 22 cell types
RNA: detected in 7 tissues Protein: detected in 57 cell types
RNA: detected in 7 tissues Protein: detected in 43 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:100
1:400
1:150
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA026726
Antibody HPA026764
Antibody CAB010907
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
144, 124.5, 124.4
144, 124.5, 124.4
144, 124.5, 124.4
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA026726
Antibody HPA026764
Antibody CAB010907
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:3000
ANTIGEN INFORMATION
Antibody HPA026726
Antibody HPA026764
Antibody CAB010907
Antigen
Recombinant protein fragment
Recombinant protein fragment
Not known
Length (aa)
101
93
Antigen sequence
GTKYIVTLRNVKNEVSSSPQHLLATTDLAVLGTAWVTDETENSLDVEWEN
PSTEVDYYKLRYGPMTGQEVAEVTVPKSSDPKSRYDITGLHPGTEYKITV
V
