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Immunohistochemical staining of human pancreas shows strong membranous and cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic and membranous positivity in exocrine glandular cells.
Immunohistochemical staining of human kidney shows strong luminal positivity in tubular cells.
Expression
RNA: detected in 24 tissues Protein: detected in 11 cell types
RNA: detected in 24 tissues Protein: detected in 56 cell types
RNA: detected in 24 tissues Protein: detected in 72 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:750
1:75
1:150
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA014722
Antibody HPA017353
Antibody CAB022569
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424464)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424464)
Target mass (kDa)
47.7
47.7
47.7
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA014722
Antibody HPA017353
Antibody CAB022569
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
