Cell atlas - TMPO - The Human Protein Atlas

GENERAL INFORMATION
Gene name	TMPO
Gene description	Thymopoietin
Protein class	Disease related genes Plasma proteins Predicted intracellular proteins Predicted membrane proteins
Predicted localization	Intracellular,Membrane
Number of transcripts	6
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the nuclear membrane.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nuclear membrane (validated)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Validated based on 2 antibodies. HPA008150 , CAB009847
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ASSAYS

RNA expression

Human cells

siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the nuclear membrane.

Main location

Nuclear membrane (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA008150	A-431	Nuclear membrane
HPA008150	U-2 OS	Nuclear membrane
HPA008150	U-251 MG	Nuclear membrane
CAB009847	A-431	Nuclear membrane
CAB009847	U-2 OS	Nuclear membrane
CAB009847	U-251 MG	Nuclear membrane

siRNA VALIDATION

Relative fluorescence intensity (RFI)

Antibody

HPA008150

siRNA validation

Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

Nuclear region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

51%

67%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Sample	Cell line
HPA008150	siRNA 2	U-2 OS
HPA008150	scrambled	U-2 OS
HPA008150	siRNA 1	U-2 OS

GENE INFORMATION
Gene name	TMPO (HGNC Symbol)
Synonyms	LAP2, LEMD4, TP
Description	Thymopoietin (HGNC Symbol)
Entrez gene summary	The protein encoded by this gene resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. Mutations in this gene are associated with dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, May 2010]
Chromosome	12
Cytoband	q23.1
Chromosome location (bp)	98515512 - 98550379
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000120802 (version 83.38)
Entrez gene	7112
UniProt	P42166 (UniProt - Evidence at protein level), P42167 (UniProt - Evidence at protein level)
neXtProt	NX_P42166, NX_P42167
Antibodypedia	TMPO antibodies

PROTEIN BROWSER

TMPO-001	TMPO-002	TMPO-005	TMPO-006	TMPO-014	TMPO-015

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
TMPO-001 ENSP00000266732 ENST00000266732	P42166 [Direct mapping] Lamina-associated polypeptide 2, isoform alpha Thymopoietin Thymopentin Show all	Predicted intracellular proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000785 [chromatin] GO:0003677 [DNA binding] GO:0005515 [protein binding] GO:0005521 [lamin binding] GO:0005634 [nucleus] GO:0005635 [nuclear envelope] GO:0005637 [nuclear inner membrane] GO:0005789 [endoplasmic reticulum membrane] GO:0006355 [regulation of transcription, DNA-templated] Show all	694 aa 75.5 kDa	No	0
TMPO-002 ENSP00000450627 ENST00000556029	P42167 [Direct mapping] Lamina-associated polypeptide 2, isoforms beta/gamma Thymopoietin Thymopentin A0A024RBE7 [Target identity:100%; Query identity:100%] Thymopoietin, isoform CRA_c Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0003677 [DNA binding] GO:0005515 [protein binding] GO:0005521 [lamin binding] GO:0005634 [nucleus] GO:0005635 [nuclear envelope] GO:0005637 [nuclear inner membrane] GO:0005737 [cytoplasm] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0031965 [nuclear membrane] Show all	454 aa 50.7 kDa	No	1
TMPO-005 ENSP00000376773 ENST00000393053	P42167 [Direct mapping] Lamina-associated polypeptide 2, isoforms beta/gamma Thymopoietin Thymopentin A0A024RBH7 [Target identity:100%; Query identity:100%] Thymopoietin, isoform CRA_a Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0003677 [DNA binding] GO:0005515 [protein binding] GO:0005521 [lamin binding] GO:0005634 [nucleus] GO:0005635 [nuclear envelope] GO:0005637 [nuclear inner membrane] GO:0005737 [cytoplasm] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0031965 [nuclear membrane] Show all	345 aa 38.7 kDa	No	1
TMPO-006 ENSP00000261210 ENST00000261210	P42167 [Direct mapping] Lamina-associated polypeptide 2, isoforms beta/gamma Thymopoietin Thymopentin Show all	Predicted intracellular proteins Plasma proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0003677 [DNA binding] GO:0005515 [protein binding] GO:0005521 [lamin binding] GO:0005634 [nucleus] GO:0005635 [nuclear envelope] GO:0005637 [nuclear inner membrane] GO:0005737 [cytoplasm] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0031965 [nuclear membrane] Show all	248 aa 27.4 kDa	No	0
TMPO-014 ENSP00000340251 ENST00000343315	G5E972 [Direct mapping] Lamina-associated polypeptide 2, isoforms beta/gamma; Thymopoietin, isoform CRA_d Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0003677 [DNA binding] GO:0016021 [integral component of membrane] Show all	414 aa 46.3 kDa	No	1
TMPO-015 ENSP00000451552 ENST00000556678	H0YJH7 [Direct mapping] Lamina-associated polypeptide 2, isoforms beta/gamma Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		237 aa 26.9 kDa	No	0