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ZNF639

ANTIBODY INFORMATION
 
 

Antibody HPA049023

  

Antibody HPA052163

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA049023   HPA052163  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA049023

  

Antibody HPA052163

  
Immunocytochemistry

Supported    
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA049023

  

Antibody HPA052163

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.    

Antibody dilution

 1:147    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049023

  

Antibody HPA052163

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.   Immunohistochemical staining of human testis shows cytoplasmic positivity in Leydig cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 64 cell types		   RNA: detected in 37 tissues
Protein: detected in 32 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:80  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA049023

  

Antibody HPA052163

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 56.1, 33, 24.5   56.1, 33, 24.5, 15.6, 14.8  

Antibody dilution

 1:730   1:80  

PROTEIN ARRAY
 
 

Antibody HPA049023

  

Antibody HPA052163

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:5900   1:650  

ANTIGEN INFORMATION
 
 

Antibody HPA049023

  

Antibody HPA052163

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 87   83  

Antigen sequence

 
SADIVICDEECDSPESVNQQTQEESPIEVHTAEDVPIAVEVHAISEDYDI
ETENNSSESLQDQTDEEPPAKLCKILDKSQALNVTAQ
  
KRKTLHPSRYSDSSGISRIADGFNGIFSDHCYSVCSMRQPDLKYFDNKDD
DSDTETSNDLPKFADGIKARNRNQNYLVPSPVL
 

Matching transcripts

 ZNF639-001 - ENSP00000325634 [100%]
ZNF639-002 - ENSP00000417740 [100%]
ZNF639-003 - ENSP00000418766 [100%]
ZNF639-004 - ENSP00000418870 [100%]
ZNF639-006 - ENSP00000419650 [100%]
ZNF639-201 - ENSP00000477626 [100%]
  ZNF639-001 - ENSP00000325634 [100%]
ZNF639-002 - ENSP00000417740 [100%]
ZNF639-003 - ENSP00000418766 [100%]
ZNF639-004 - ENSP00000418870 [100%]
ZNF639-005 - ENSP00000418628 [100%]
ZNF639-006 - ENSP00000419650 [100%]
ZNF639-007 - ENSP00000417232 [100%]
ZNF639-201 - ENSP00000477626 [100%]
 

Other gene match

    
ANTIGEN VIEW
ZNF639-001
ZNF639-002
ZNF639-003
ZNF639-004
ZNF639-005
ZNF639-006
ZNF639-007
ZNF639-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.