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Antibody staining overlaps with antibody HPA043544.
Validated
Antibody staining overlaps with antibody HPA041725.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles.
Antibody dilution
1:200
1:34
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA041725
Antibody HPA043544
Antibody CAB032866
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 52 cell types
RNA: detected in 37 tissues Protein: detected in 12 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:500
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041725
Antibody HPA043544
Antibody CAB032866
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
201.6, 197.3, 106
201.6, 197.3, 106
201.6, 197.3, 113.5, 106, 33, 17.3, 15, 13.2
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA041725
Antibody HPA043544
Antibody CAB032866
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
