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ANTIBODY INFORMATION
 
 

Antibody HPA056386

  

Antibody CAB032738

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA056386   sc-30171  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 12   7  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA056386

  

Antibody CAB032738

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA056386

  

Antibody CAB032738

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in spermatids and spermatozoa.   Immunohistochemical staining of human testis shows strong nuclear positivity in subsets of cells in seminiferus ducts.  

Expression

 RNA: detected in 3 tissues
Protein: detected in 6 cell types		   RNA: detected in 3 tissues
Protein: detected in 12 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:150  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA056386

  

Antibody CAB032738

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 17.1, 13.1   17.1, 13.1  

Antibody dilution

 1:400   1:500  

PROTEIN ARRAY
 
 

Antibody HPA056386

  

Antibody CAB032738

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:4800    

ANTIGEN INFORMATION
 
 

Antibody HPA056386

  

Antibody CAB032738

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 51    

Antigen sequence

 
MVRYRVRSLSERSHEVYRQQLHGQEQGHHGQEEQGLSPEHVEVYERTHGQ
S
   

Matching transcripts

 PRM2-001 - ENSP00000241808 [100%]
PRM2-002 - ENSP00000403681 [100%]
   

Other gene match

    
ANTIGEN VIEW
PRM2-001
PRM2-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA056386

  

Antibody CAB032738

  

1.

 The human testis-specific proteome defined by transcriptomics and antibody-based profiling
Djureinovic D et al
Mol Hum Reprod 2014;20(6):476-88
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.