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FAM213A

ANTIBODY INFORMATION
 
 

Antibody HPA009025

  

Antibody HPA024565

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA009025   HPA024565  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   5  

References

 3    

VALIDATION SUMMARY
 
 

Antibody HPA009025

  

Antibody HPA024565

  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA009025

  

Antibody HPA024565

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human breast shows strong cytoplasmic positivity in adipocytes.   Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in adipocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 71 cell types		   RNA: detected in 37 tissues
Protein: detected in 33 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:20  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA009025

  

Antibody HPA024565

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: Human Plasma
Lane 4: U-251 MG
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 25.8, 24.5   25.8, 24.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA009025

  

Antibody HPA024565

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA009025

  

Antibody HPA024565

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 99   90  

Antigen sequence

 
NTDVFLSKPQKAALEYLEDIDLKTLEKEPRTFKAKELWEKNGAVIMAVRR
PGCFLCREEAADLSSLKSMLDQLGVPLYAVVKEHIRTEVKDFQPYFKGE
  
KFYGPQRRKMMFMGFIRLGVWYNFFRAWNGGFSGNLEGEGFILGGVFVVG
SGKQGILLEHREKEFGDKVNLLSVLEAAKMIKPQTLASEK
 

Matching transcripts

 FAM213A-001 - ENSP00000482445 [100%]
FAM213A-002 - ENSP00000361259 [100%]
FAM213A-003 - ENSP00000361262 [100%]
FAM213A-004 - ENSP00000361254 [100%]
FAM213A-201 - ENSP00000361261 [100%]
FAM213A-202 - ENSP00000478152 [100%]
  FAM213A-001 - ENSP00000482445 [100%]
FAM213A-002 - ENSP00000361259 [100%]
FAM213A-003 - ENSP00000361262 [100%]
FAM213A-004 - ENSP00000361254 [100%]
FAM213A-201 - ENSP00000361261 [100%]
FAM213A-202 - ENSP00000478152 [100%]
 

Other gene match

    
ANTIGEN VIEW
FAM213A-001
FAM213A-002
FAM213A-003
FAM213A-004
FAM213A-201
FAM213A-202

RELEVANT PUBLICATIONS
 
 

Antibody HPA009025

  

Antibody HPA024565

  

1.

 PAMM: a redox regulatory protein that modulates osteoclast differentiation
Xu Y et al
Antioxid Redox Signal 2010;13(1):27-37
    

2.

 Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C et al
J Proteomics 2012;75(7):2236-51
    

3.

 A short report: PAMM, a novel antioxidant protein, induced by oxidative stress
Xu Y et al
Redox Biol 2015;6:446-53
Application: IHC, WB 		   
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.