Fields »
ABOUT
HELP
BLOG
POLM

ANTIBODY INFORMATION
 
 

Antibody HPA058900

  

Antibody HPA066007

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA058900   HPA066007  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 13   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA058900

  

Antibody HPA066007

  
Immunocytochemistry

Approved   Approved  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA058900

  

Antibody HPA066007

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & cytosol.  

Antibody dilution

 1:67   1:92  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA058900

  

Antibody HPA066007

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 54.8   55.1, 54.8, 50.4, 17.7  

Antibody dilution

 1:220   1:310  

PROTEIN ARRAY
 
 

Antibody HPA058900

  

Antibody HPA066007

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2700   1:3700  

ANTIGEN INFORMATION
 
 

Antibody HPA058900

  

Antibody HPA066007

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 94   92  

Antigen sequence

 
ITHPKEGQEAGLLPRVMCRLQDQGLILYHQHQHSCCESPTRLAQQSHMDA
FERSFCIFRLPQPPGAAVGGSTRPCPSWKAVRVDLVVAPVSQFP
  
SEATHVVMEETSAEEAVSWQERRMAAAPPGCTPPALLDISWLTESLGAGQ
PVPVECRHRLEVAGPRKGPLSPAWMPAYACQRPTPLTHHNTG
 

Matching transcripts

 POLM-002 - ENSP00000242248 [100%]
  POLM-002 - ENSP00000242248 [100%]
POLM-006 - ENSP00000379174 [100%]
POLM-010 - ENSP00000335141 [100%]
POLM-011 - ENSP00000399244 [100%]
 

Other gene match

    
ANTIGEN VIEW
POLM-002
POLM-006
POLM-010
POLM-011
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.