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Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in cells of the seminiferus ducts.
Immunohistochemical staining of human testis shows cytoplasmic positivity in cells of seminiferus ducts.
Expression
RNA: detected in 5 tissues Protein: detected in 16 cell types
RNA: detected in 5 tissues Protein: detected in 9 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:550
1:900
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA021649
Antibody HPA021843
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402158)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402158)
Target mass (kDa)
79.8, 79.3, 32.7
79.8, 79.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA021649
Antibody HPA021843
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
