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Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & the Golgi apparatus.
Antibody dilution
1:164
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA047523
Antibody HPA055704
Standard validation
Approved
Figure description
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.
Expression
RNA: detected in 33 tissues Protein: detected in 29 cell types
Retrieval
HIER pH6
Antibody dilution
1:300
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047523
Antibody HPA055704
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
54.7
54.7
Antibody dilution
1:810
1:420
PROTEIN ARRAY
Antibody HPA047523
Antibody HPA055704
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:6500
1:5000
ANTIGEN INFORMATION
Antibody HPA047523
Antibody HPA055704
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
51
51
Antigen sequence
IISRVSDKLKQIPQALADANSTDPALILAENASLLSLSELDSAFSQLQSR
L
IISRVSDKLKQIPQALADANSTDPALILAENASLLSLSELDSAFSQLQSR
L
