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Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Antibody dilution
1:20
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA023180
Standard validation
Approved
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells and moderate nuclear membrane staining in islet cells.
Expression
RNA: detected in 37 tissues Protein: detected in 76 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023180
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Target mass (kDa)
105.2
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA023180
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
