DBH - Antibodies - The Human Protein Atlas

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DBH

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA002130

Antibody HPA005960

Antibody HPA070789

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA002130

HPA005960

HPA070789

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA002130

Antibody HPA005960

Antibody HPA070789

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA002130

Antibody HPA005960

Antibody HPA070789

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.

Antibody dilution

1:8

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA002130

Antibody HPA005960

Antibody HPA070789

Standard validation

Supported

Figure description

Immunohistochemical staining of adrenal gland shows cytoplasmic positivity in medullary cells.

Immunohistochemical staining of adrenal gland shows strong cytoplasmic positivity in medullary cells.

Expression

RNA: detected in 11 tissues
Protein: detected in 4 cell types

RNA: detected in 11 tissues
Protein: detected in 6 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:125

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA002130

Antibody HPA005960

Antibody HPA070789

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

69.1

Antibody dilution

1:250

1:80

PROTEIN ARRAY

Antibody HPA002130

Antibody HPA005960

Antibody HPA070789

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:2000

1:500

ANTIGEN INFORMATION

Antibody HPA002130

Antibody HPA005960

Antibody HPA070789

Antigen

Recombinant protein fragment

Length (aa)

122

Antigen sequence

VQRTPEGLTLLFKRPFGTCDPKDYLIEDGTVHLVYGILEEPFRSLEAING
SGLQMGLQRVQLLKPNIPEPELPSDACTMEVQAPNIQIPSQETTYWCYIK
ELPKGFSRHHIIKYEPIVTKGN

VQRTPEGLTLLFKRPFGTCDPKDYLIEDGTVHLVYGILEEPFRSLEAING
SGLQMGLQRVQLLKPNIPEPELPSDACTMEVQAPNIQIPSQETTYWCYIK
ELPKGFSRHHIIKYEPIVTKGN

LINRFNNEDVCTCPQASVSQQFTSVPWNSFNRDVLKALYSFAPISMHCNK
SSAVRFQGEWNLQPLPKVISTLEEPTPQCPTSQ

Matching transcripts

DBH-001 - ENSP00000376776 [100%]

Other gene match

ANTIGEN VIEW

DBH-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.