Fields »
ABOUT
HELP
BLOG
CKS2

ANTIBODY INFORMATION
 
 

Antibody HPA003424

  

Antibody HPA030762

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA003424   HPA030762  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 CKS1B - ENSG00000173207 [82%]
   CKS1B - ENSG00000173207 [82%]
  

Released in version

 16   7  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA003424

  

Antibody HPA030762

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Approved  
Western blot

  Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003424

  

Antibody HPA030762

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line A549 shows localization to cytosol & mitochondria.    

Antibody dilution

 1:32    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003424

  

Antibody HPA030762

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells and non-germinal center cells.  

Expression

   RNA: detected in 36 tissues
Protein: detected in 82 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:150  

Literature conformity

   Partly consistent with gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  
 

Standard validation

 Supported    

   

Figure description

 microglia, neurons and endothelial cells in specific regions.    

WESTERN BLOT
 
 

Antibody HPA003424

  

Antibody HPA030762

  

Standard validation

   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

   

Figure description

    

Target mass (kDa)

   9.9, 9.7  

Antibody dilution

   1:250  

PROTEIN ARRAY
 
 

Antibody HPA003424

  

Antibody HPA030762

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA003424

  

Antibody HPA030762

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 76   76  

Antigen sequence

 
HKQIYYSDKYFDEHYEYRHVMLPRELSKQVPKTHLMSEEEWRRLGVQQSL
GWVHYMIHEPEPHILLFRRPLPKDQQ
  
HKQIYYSDKYFDEHYEYRHVMLPRELSKQVPKTHLMSEEEWRRLGVQQSL
GWVHYMIHEPEPHILLFRRPLPKDQQ
 

Matching transcripts

 CKS2-001 - ENSP00000364976 [100%]
  CKS2-001 - ENSP00000364976 [100%]
 

Other gene match

 CKS1B - ENSG00000173207 [82%]
   CKS1B - ENSG00000173207 [82%]
  
ANTIGEN VIEW
CKS2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.