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Antibody staining overlaps with antibody HPA042033.
Validated
Antibody staining overlaps with antibody HPA041019.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:139
1:56
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA041019
Antibody HPA042033
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human lateral ventricle shows moderate cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 60 cell types
RNA: detected in 37 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:500
1:100
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041019
Antibody HPA042033
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418449)
