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Immunofluorescent staining of human cell line SiHa shows localization to vesicles.
Antibody dilution
1:133
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA047417
Antibody CAB011618
Antibody CAB070164
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human duodenum shows distinct cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 68 cell types
RNA: detected in 37 tissues Protein: detected in 40 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:450
1:900
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047417
Antibody CAB011618
Antibody CAB070164
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
292.3
292.3, 290.5
292.3, 290.5
Antibody dilution
1:660
1:500
1:500
PROTEIN ARRAY
Antibody HPA047417
Antibody CAB011618
Antibody CAB070164
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
