TRERF1 - Antibodies - The Human Protein Atlas

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TRERF1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA027771

Antibody HPA051273

Antibody HPA059943

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA027771

HPA051273

HPA059943

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA027771

Antibody HPA051273

Antibody HPA059943

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA027771

Antibody HPA051273

Antibody HPA059943

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Antibody dilution

1:43

1:104

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA027771

Antibody HPA051273

Antibody HPA059943

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Expression

RNA: detected in 33 tissues
Protein: detected in 48 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA027771

Antibody HPA051273

Antibody HPA059943

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

134.3, 132.3, 125, 123.6, 106.3

Antibody dilution

1:250

1:520

1:260

PROTEIN ARRAY

Antibody HPA027771

Antibody HPA051273

Antibody HPA059943

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:4150

1:3100

ANTIGEN INFORMATION

Antibody HPA027771

Antibody HPA051273

Antibody HPA059943

Antigen

Recombinant protein fragment

Length (aa)

116

Antigen sequence

QGSGLFSNVLISGHGPGAHPQLPLTPLTPTPRVLLCRSNSIDGSNVTVTP
GPGEQTVDVEPRINIGLRFQAEIPELQDISALAQDTHKATLVWKPWPELE
NHDLQQRVENLLNLCC

PSGIHLNNMGPQHQQLSPSAMWPQMHLPDGRAQPGSPESSGQPKGAFGEQ
FDAKNKLTCSICLKEFKNLPALNGHMRSHG

PSGIHLNNMGPQHQQLSPSAMWPQMHLPDGRAQPGSPESSGQPKGAFGEQ
FDAKNKLTCSICLKEFKNLPALNGHMRSHG

Matching transcripts

TRERF1-001 - ENSP00000362013 [100%]
TRERF1-002 - ENSP00000339438 [100%]
TRERF1-003 - ENSP00000346285 [100%]
TRERF1-201 - ENSP00000362008 [100%]
TRERF1-202 - ENSP00000439689 [100%]

Other gene match

ANTIGEN VIEW

TRERF1-001	TRERF1-002	TRERF1-003	TRERF1-201	TRERF1-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.