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CDKN1A

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GENERAL INFORMATION

Gene name

 CDKN1A

Gene description

 Cyclin-dependent kinase inhibitor 1A (p21, Cip1)

Protein class

 Cancer-related genes
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 5
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Localized to the nuclear bodies & nucleoplasm.

RNA cell category

 Cell line enhanced (hTCEpi, LHCN-M2)

Protein evidence

 Evidence at protein level

Main location

 Localized to the Nucleoplasm (supported), Nuclear bodies (supported)

Single-cell variation

 Single-cell variation in protein expression observed.

Cell cycle dependency

 Cell cycle dependent expression determined by custom assay

DATA RELIABILITY

Reliability score

 Supported based on 1 antibody.
HPA005946
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ASSAYS
RNA expression
Human cells
Custom data

RNA EXPRESSION OVERVIEW
RNA cell line category: Cell line enhanced (hTCEpi, LHCN-M2)
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Localized to the nuclear bodies & nucleoplasm.

Main location

 Nucleoplasm (supported), Nuclear bodies (supported)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
HPA005946 BJ Nucleoplasm Spatial
Nuclear bodies Spatial
HPA005946 RT4 Nucleoplasm Intensity
HPA005946 U-2 OS Nucleoplasm

CUSTOM DATA

 

 

 
Toggle channels

Thumbnail

Antibody

Cell line

Assay

Description
HPA005946 U-2 OS Image Staining of U-2 OS FUCCI cells, to characterize the cell cycle dependency of the protein expression pattern. The FUCCI cells, Fluorescence Ubiquitination Cell Cycle Indicator, are cells tagged with different fluorescent proteins fused to two different cell cycle regulators Cdt1 (expressed in G1 phase) and Geminin (expressed in S and G2 phases) that allows cell cycle monitoring. When both proteins are present, the overlay of the images appear in yellow marking the G1/S transition. CDKN1A is mostly expressed during G1, and during S and/or G2.

CELL CYCLE

Summary

 Expression has cell cycle dependency according to custom assay

Spatial variation

 Nuclear bodies, Nucleoplasm

GENE INFORMATION

Gene name

 CDKN1A (HGNC Symbol)

Synonyms

 CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1

Description

 Cyclin-dependent kinase inhibitor 1A (p21, Cip1) (HGNC Symbol)

Entrez gene summary

 This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2015]

Chromosome

 6

Cytoband

 p21.2

Chromosome location (bp)

 36676460 - 36687339

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000124762 (version 83.38)

Entrez gene

 1026

UniProt

 P38936 (UniProt - Evidence at protein level)

neXtProt

 NX_P38936

Antibodypedia

 CDKN1A antibodies

PROTEIN BROWSER
CDKN1A-001
CDKN1A-002
CDKN1A-201
CDKN1A-202
CDKN1A-203

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
CDKN1A-001
ENSP00000244741
ENST00000244741
P38936 [Direct mapping]
Cyclin-dependent kinase inhibitor 1
A0A024RCX5 [Target identity:100%; Query identity:100%]
Cyclin-dependent kinase inhibitor 1A (P21, Cip1), isoform CRA_a
Show all
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
   Mutated cancer genes
   Mutational cancer driver genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000079 [regulation of cyclin-dependent protein serine/threonine kinase activity]
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000086 [G2/M transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0000307 [cyclin-dependent protein kinase holoenzyme complex]
GO:0004861 [cyclin-dependent protein serine/threonine kinase inhibitor activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005829 [cytosol]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0006977 [DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest]
GO:0007050 [cell cycle arrest]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007265 [Ras protein signal transduction]
GO:0008285 [negative regulation of cell proliferation]
GO:0008543 [fibroblast growth factor receptor signaling pathway]
GO:0019912 [cyclin-dependent protein kinase activating kinase activity]
GO:0030308 [negative regulation of cell growth]
GO:0031625 [ubiquitin protein ligase binding]
GO:0031668 [cellular response to extracellular stimulus]
GO:0038095 [Fc-epsilon receptor signaling pathway]
GO:0042326 [negative regulation of phosphorylation]
GO:0043231 [intracellular membrane-bounded organelle]
GO:0045087 [innate immune response]
GO:0046872 [metal ion binding]
GO:0048011 [neurotrophin TRK receptor signaling pathway]
GO:0048015 [phosphatidylinositol-mediated signaling]
GO:0048146 [positive regulation of fibroblast proliferation]
GO:0070557 [PCNA-p21 complex]
GO:0071479 [cellular response to ionizing radiation]
GO:0071493 [cellular response to UV-B]
GO:0090398 [cellular senescence]
GO:0090400 [stress-induced premature senescence]
GO:0097193 [intrinsic apoptotic signaling pathway]
GO:1904031 [positive regulation of cyclin-dependent protein kinase activity]
GO:2000134 [negative regulation of G1/S transition of mitotic cell cycle]
GO:2000379 [positive regulation of reactive oxygen species metabolic process]
Show all
 164 aa
18.1 kDa 		No 0
CDKN1A-002
ENSP00000362815
ENST00000373711
P38936 [Direct mapping]
Cyclin-dependent kinase inhibitor 1
A0A024RCX5 [Target identity:100%; Query identity:100%]
Cyclin-dependent kinase inhibitor 1A (P21, Cip1), isoform CRA_a
Show all
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
   Mutated cancer genes
   Mutational cancer driver genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000079 [regulation of cyclin-dependent protein serine/threonine kinase activity]
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000086 [G2/M transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0000307 [cyclin-dependent protein kinase holoenzyme complex]
GO:0004861 [cyclin-dependent protein serine/threonine kinase inhibitor activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005829 [cytosol]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0006977 [DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest]
GO:0007050 [cell cycle arrest]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007265 [Ras protein signal transduction]
GO:0008285 [negative regulation of cell proliferation]
GO:0008543 [fibroblast growth factor receptor signaling pathway]
GO:0019912 [cyclin-dependent protein kinase activating kinase activity]
GO:0030308 [negative regulation of cell growth]
GO:0031625 [ubiquitin protein ligase binding]
GO:0031668 [cellular response to extracellular stimulus]
GO:0038095 [Fc-epsilon receptor signaling pathway]
GO:0042326 [negative regulation of phosphorylation]
GO:0043231 [intracellular membrane-bounded organelle]
GO:0045087 [innate immune response]
GO:0046872 [metal ion binding]
GO:0048011 [neurotrophin TRK receptor signaling pathway]
GO:0048015 [phosphatidylinositol-mediated signaling]
GO:0048146 [positive regulation of fibroblast proliferation]
GO:0070557 [PCNA-p21 complex]
GO:0071479 [cellular response to ionizing radiation]
GO:0071493 [cellular response to UV-B]
GO:0090398 [cellular senescence]
GO:0090400 [stress-induced premature senescence]
GO:0097193 [intrinsic apoptotic signaling pathway]
GO:1904031 [positive regulation of cyclin-dependent protein kinase activity]
GO:2000134 [negative regulation of G1/S transition of mitotic cell cycle]
GO:2000379 [positive regulation of reactive oxygen species metabolic process]
Show all
 164 aa
18.1 kDa 		No 0
CDKN1A-201
ENSP00000384849
ENST00000405375
P38936 [Direct mapping]
Cyclin-dependent kinase inhibitor 1
A0A024RCX5 [Target identity:100%; Query identity:100%]
Cyclin-dependent kinase inhibitor 1A (P21, Cip1), isoform CRA_a
Show all
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
   Mutated cancer genes
   Mutational cancer driver genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000079 [regulation of cyclin-dependent protein serine/threonine kinase activity]
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000086 [G2/M transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0000307 [cyclin-dependent protein kinase holoenzyme complex]
GO:0004861 [cyclin-dependent protein serine/threonine kinase inhibitor activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0006977 [DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest]
GO:0007050 [cell cycle arrest]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007265 [Ras protein signal transduction]
GO:0007346 [regulation of mitotic cell cycle]
GO:0008285 [negative regulation of cell proliferation]
GO:0008543 [fibroblast growth factor receptor signaling pathway]
GO:0009411 [response to UV]
GO:0009636 [response to toxic substance]
GO:0010033 [response to organic substance]
GO:0010165 [response to X-ray]
GO:0010243 [response to organonitrogen compound]
GO:0010629 [negative regulation of gene expression]
GO:0010942 [positive regulation of cell death]
GO:0014070 [response to organic cyclic compound]
GO:0019912 [cyclin-dependent protein kinase activating kinase activity]
GO:0030308 [negative regulation of cell growth]
GO:0030332 [cyclin binding]
GO:0030890 [positive regulation of B cell proliferation]
GO:0031100 [organ regeneration]
GO:0031625 [ubiquitin protein ligase binding]
GO:0031668 [cellular response to extracellular stimulus]
GO:0032403 [protein complex binding]
GO:0033158 [regulation of protein import into nucleus, translocation]
GO:0034605 [cellular response to heat]
GO:0038095 [Fc-epsilon receptor signaling pathway]
GO:0042326 [negative regulation of phosphorylation]
GO:0042493 [response to drug]
GO:0042771 [intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator]
GO:0043066 [negative regulation of apoptotic process]
GO:0043068 [positive regulation of programmed cell death]
GO:0043231 [intracellular membrane-bounded organelle]
GO:0045087 [innate immune response]
GO:0045736 [negative regulation of cyclin-dependent protein serine/threonine kinase activity]
GO:0046685 [response to arsenic-containing substance]
GO:0046872 [metal ion binding]
GO:0048011 [neurotrophin TRK receptor signaling pathway]
GO:0048015 [phosphatidylinositol-mediated signaling]
GO:0048146 [positive regulation of fibroblast proliferation]
GO:0048471 [perinuclear region of cytoplasm]
GO:0051384 [response to glucocorticoid]
GO:0051412 [response to corticosterone]
GO:0051726 [regulation of cell cycle]
GO:0055093 [response to hyperoxia]
GO:0060574 [intestinal epithelial cell maturation]
GO:0070557 [PCNA-p21 complex]
GO:0071479 [cellular response to ionizing radiation]
GO:0071493 [cellular response to UV-B]
GO:0071850 [mitotic cell cycle arrest]
GO:0090398 [cellular senescence]
GO:0090399 [replicative senescence]
GO:0090400 [stress-induced premature senescence]
GO:0097193 [intrinsic apoptotic signaling pathway]
GO:1904031 [positive regulation of cyclin-dependent protein kinase activity]
GO:2000134 [negative regulation of G1/S transition of mitotic cell cycle]
GO:2000278 [regulation of DNA biosynthetic process]
GO:2000379 [positive regulation of reactive oxygen species metabolic process]
Show all
 164 aa
18.1 kDa 		No 0
CDKN1A-202
ENSP00000409259
ENST00000448526
P38936 [Direct mapping]
Cyclin-dependent kinase inhibitor 1
A0A024RCX5 [Target identity:100%; Query identity:100%]
Cyclin-dependent kinase inhibitor 1A (P21, Cip1), isoform CRA_a
Show all
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
   Mutated cancer genes
   Mutational cancer driver genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000079 [regulation of cyclin-dependent protein serine/threonine kinase activity]
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000086 [G2/M transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0000307 [cyclin-dependent protein kinase holoenzyme complex]
GO:0004861 [cyclin-dependent protein serine/threonine kinase inhibitor activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005829 [cytosol]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0006977 [DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest]
GO:0007050 [cell cycle arrest]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007265 [Ras protein signal transduction]
GO:0008285 [negative regulation of cell proliferation]
GO:0008543 [fibroblast growth factor receptor signaling pathway]
GO:0019912 [cyclin-dependent protein kinase activating kinase activity]
GO:0030308 [negative regulation of cell growth]
GO:0031625 [ubiquitin protein ligase binding]
GO:0031668 [cellular response to extracellular stimulus]
GO:0038095 [Fc-epsilon receptor signaling pathway]
GO:0042326 [negative regulation of phosphorylation]
GO:0043231 [intracellular membrane-bounded organelle]
GO:0045087 [innate immune response]
GO:0046872 [metal ion binding]
GO:0048011 [neurotrophin TRK receptor signaling pathway]
GO:0048015 [phosphatidylinositol-mediated signaling]
GO:0048146 [positive regulation of fibroblast proliferation]
GO:0070557 [PCNA-p21 complex]
GO:0071479 [cellular response to ionizing radiation]
GO:0071493 [cellular response to UV-B]
GO:0090398 [cellular senescence]
GO:0090400 [stress-induced premature senescence]
GO:0097193 [intrinsic apoptotic signaling pathway]
GO:1904031 [positive regulation of cyclin-dependent protein kinase activity]
GO:2000134 [negative regulation of G1/S transition of mitotic cell cycle]
GO:2000379 [positive regulation of reactive oxygen species metabolic process]
Show all
 164 aa
18.1 kDa 		No 0
CDKN1A-203
ENSP00000482768
ENST00000615513
P38936 [Direct mapping]
Cyclin-dependent kinase inhibitor 1
A0A024RCX5 [Target identity:100%; Query identity:100%]
Cyclin-dependent kinase inhibitor 1A (P21, Cip1), isoform CRA_a
Show all
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
   Mutated cancer genes
   Mutational cancer driver genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000079 [regulation of cyclin-dependent protein serine/threonine kinase activity]
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000086 [G2/M transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0000307 [cyclin-dependent protein kinase holoenzyme complex]
GO:0004861 [cyclin-dependent protein serine/threonine kinase inhibitor activity]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005829 [cytosol]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0006977 [DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest]
GO:0007050 [cell cycle arrest]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007265 [Ras protein signal transduction]
GO:0008285 [negative regulation of cell proliferation]
GO:0008543 [fibroblast growth factor receptor signaling pathway]
GO:0019912 [cyclin-dependent protein kinase activating kinase activity]
GO:0030308 [negative regulation of cell growth]
GO:0031625 [ubiquitin protein ligase binding]
GO:0031668 [cellular response to extracellular stimulus]
GO:0038095 [Fc-epsilon receptor signaling pathway]
GO:0042326 [negative regulation of phosphorylation]
GO:0043231 [intracellular membrane-bounded organelle]
GO:0045087 [innate immune response]
GO:0046872 [metal ion binding]
GO:0048011 [neurotrophin TRK receptor signaling pathway]
GO:0048015 [phosphatidylinositol-mediated signaling]
GO:0048146 [positive regulation of fibroblast proliferation]
GO:0070557 [PCNA-p21 complex]
GO:0071479 [cellular response to ionizing radiation]
GO:0071493 [cellular response to UV-B]
GO:0090398 [cellular senescence]
GO:0090400 [stress-induced premature senescence]
GO:0097193 [intrinsic apoptotic signaling pathway]
GO:1904031 [positive regulation of cyclin-dependent protein kinase activity]
GO:2000134 [negative regulation of G1/S transition of mitotic cell cycle]
GO:2000379 [positive regulation of reactive oxygen species metabolic process]
Show all
 164 aa
18.1 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.