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RREB1

ANTIBODY INFORMATION
 
 

Antibody HPA001756

  

Antibody HPA034843

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001756   HPA034843  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   12  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA001756

  

Antibody HPA034843

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001756

  

Antibody HPA034843

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & nucleoli fibrillar center.  

Antibody dilution

   1:47  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001756

  

Antibody HPA034843

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human duodenum shows distinct cytoplasmic and nuclear positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 57 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:35    

Literature conformity

 Partly consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA001756

  

Antibody HPA034843

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 187.6, 159.3   187.6, 181.4, 159.3, 79.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA001756

  

Antibody HPA034843

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA001756

  

Antibody HPA034843

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 125   91  

Antigen sequence

 
ATTDTNKFSPFLQTAEDNTQDEVAGAPADHHGPSDEEQGSPPEDKLLRAK
RNSYTNCLQKITCPHCPRVFPWASSLQRHMLTHTGQKPFPCQKCDAFFST
KSNCERHQLRKHGVTIRRAPPLSNN
  
GSVPKAATTATPAATTSPKESSEPPAPASSPEAASPTEQGPAGTSKKRGR
KRGMRSRPRANSGGVDLDSSGEFASIEKMLATTDTNKFSPF
 

Matching transcripts

 RREB1-001 - ENSP00000369270 [92%]
RREB1-003 - ENSP00000335574 [92%]
  RREB1-001 - ENSP00000369270 [100%]
RREB1-002 - ENSP00000305560 [100%]
RREB1-003 - ENSP00000335574 [100%]
RREB1-004 - ENSP00000369265 [100%]
RREB1-201 - ENSP00000483108 [100%]
 

Other gene match

    
ANTIGEN VIEW
RREB1-001
RREB1-002
RREB1-003
RREB1-004
RREB1-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA001756

  

Antibody HPA034843

  

1.

 RREB1 transcription factor splice variants in urologic cancer
Nitz MD et al
Am J Pathol 2011;179(1):477-86
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.