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AHNAK

ANTIBODY INFORMATION
 
 

Antibody HPA019010

  

Antibody HPA019070

  

Antibody HPA026643

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA019010   HPA019070   HPA026643  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 4   4   7  

References

 1   2    

VALIDATION SUMMARY
 
 

Antibody HPA019010

  

Antibody HPA019070

  

Antibody HPA026643

  
Immunocytochemistry


Validated
  

Validated
   Uncertain  
Immunohistochemistry

Uncertain   Uncertain   Uncertain  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019010

  

Antibody HPA019070

  

Antibody HPA026643

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA019070.		  
Validated

Antibody staining overlaps with antibody HPA019010.		    
 

Standard validation

 Approved   Approved   Uncertain  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & vesicles.  

Antibody dilution

 1:9   1:56   1:20  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019010

  

Antibody HPA019070

  

Antibody HPA026643

  

Standard validation

 Uncertain   Uncertain   Uncertain  

     

Figure description

 Immunohistochemical staining of human oral mucosa shows cytoplasmic positivity in squamous epithelial cells.   Immunohistochemical staining of human rectum shows cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human small intestine shows strong membranous and cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 36 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		   RNA: detected in 37 tissues
Protein: detected in 72 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:500   1:250  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019010

  

Antibody HPA019070

  

Antibody HPA026643

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

     

Figure description

      

Target mass (kDa)

 629.1   629.1   629.1  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA019010

  

Antibody HPA019070

  

Antibody HPA026643

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA019010

  

Antibody HPA019070

  

Antibody HPA026643

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 134   124   132  

Antigen sequence

 
LGEGHLSVKGSGGEWKGPQVSSALNLDTSKFAGGLHFSGPKVEGGVKGGQ
IGLQAPGLSVSGPQGHLESGSGKVTFPKMKIPKFTFSGRELVGREMGVDV
HFPKAEASIQAGAGDGEWEESEVKLKKSKIKMPK
  
DIKSPSLDVTVPEAELNLETPEISVGGKGKKSKFKMPKIHMSGPKIKAKK
QGFDLNVPGGEIDASLKAPDVDVNIAGPDAALKVDVKSPKTKKTMFGKMY
FPDVEFDIKSPKFKAEAPLPSPKL
  
MKGTKVKTPEMIIQKPKISMQDVDLSLGSPKLKGDIKVSAPGVQGDVKGP
QVALKGSRVDIETPNLEGTLTGPRLGSPSGKTGTCRISMSEVDLNVAAPK
VKGGVDVTLPRVEGKVKVPEVDVRGPKVDVSA
 

Matching transcripts

 AHNAK-005 - ENSP00000367263 [100%]
  AHNAK-005 - ENSP00000367263 [100%]
  AHNAK-005 - ENSP00000367263 [100%]
 

Other gene match

      
ANTIGEN VIEW
AHNAK-005

RELEVANT PUBLICATIONS
 
 

Antibody HPA019010

  

Antibody HPA019070

  

Antibody HPA026643

  

1.

 Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP)
Fagerberg L et al
J Proteome Res 2013;12(6):2439-48
   AHNAK and inflammatory markers predict poor survival in laryngeal carcinoma
Dumitru CA et al
PLoS One 2013;8(2):e56420
    

2.

   Contribution of antibody-based protein profiling to the human Chromosome-centric Proteome Project (C-HPP)
Fagerberg L et al
J Proteome Res 2013;12(6):2439-48
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.