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Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & vesicles.
Antibody dilution
1:46
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA049627
Standard validation
Approved
Figure description
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in a subset of glandular cells.
Expression
RNA: detected in 19 tissues Protein: detected in 50 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA049627
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
70.6, 68.6
Antibody dilution
1:230
PROTEIN ARRAY
Antibody HPA049627
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
