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ANTIBODY INFORMATION
 
 

Antibody HPA041071

  

Antibody HPA044233

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041071   HPA044233  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   9  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA041071

  

Antibody HPA044233

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041071

  

Antibody HPA044233

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to centrosome.  

Antibody dilution

   1:33  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041071

  

Antibody HPA044233

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 75 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:20   1:75  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041071

  

Antibody HPA044233

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 62.3   62.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041071

  

Antibody HPA044233

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA041071

  

Antibody HPA044233

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 87   92  

Antigen sequence

 
ALESVVGSLEHDGGEILTDMKYGHLWSVQADSPCVANWPDLLSQCGCGLY
NSQEELNWSFLRSTRRPFVPQSCLPHEAVGSASNLTL
  
KSTALKVALFCTTLSGDTSDTGEGTVVVSYGVSLENAVLDQLLNLGRQLI
SDHVDLVLCQKVIHPSLKQFLNMHRIIAIDRIGVTLMEPLTK
 

Matching transcripts

 MKKS-001 - ENSP00000246062 [100%]
MKKS-002 - ENSP00000382008 [100%]
  MKKS-001 - ENSP00000246062 [100%]
MKKS-002 - ENSP00000382008 [100%]
 

Other gene match

    
ANTIGEN VIEW
MKKS-001
MKKS-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.