PCNXL4 - Antibodies - The Human Protein Atlas

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PCNXL4

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA002076

Antibody HPA003390

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA002076

HPA003390

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA002076

Antibody HPA003390

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA002076

Antibody HPA003390

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Antibody dilution

1:125

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA002076

Antibody HPA003390

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.

Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 50 cell types

RNA: detected in 37 tissues
Protein: detected in 76 cell types

Retrieval

HIER pH6

Antibody dilution

1:50

1:400

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA002076

Antibody HPA003390

Formal validation: Genetic

Validated

Downregulation visible in one of two siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

132.7, 106.3

Loading control

Total protein image

Antibody dilution

1:61

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

132.7, 106.3

132.7, 25.9

Antibody dilution

1:500

1:250

PROTEIN ARRAY

Antibody HPA002076

Antibody HPA003390

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA002076

Antibody HPA003390

Antigen

Recombinant protein fragment

Length (aa)

126

Antigen sequence

ELIKYLEEYERDWYIGLVSDEKWKEAILQEKPYLFSLGYDSNMGIYTGRV
LSLQELLIQVGKLNPEAVRGQWANLSWELLYATNDDEERYSIQAHPLLLR
NLTVQAAEPPLGYPIYSSKPLHIHLY

NKSTTVERILTTDILAEEDEHEFTSCTGAETVKFLIPGKKYV

Matching transcripts

PCNXL4-003 - ENSP00000317396 [100%]
PCNXL4-005 - ENSP00000384801 [100%]

PCNXL4-004 - ENSP00000375469 [100%]
PCNXL4-005 - ENSP00000384801 [100%]

Other gene match

ANTIGEN VIEW

PCNXL4-003	PCNXL4-004	PCNXL4-005

RELEVANT PUBLICATIONS

Antibody HPA002076

Antibody HPA003390

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.