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ANTIBODY INFORMATION
 
 

Antibody HPA041403

  

Antibody HPA041471

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041403   HPA041471  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   8  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA041403

  

Antibody HPA041471

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041403

  

Antibody HPA041471

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human kidney shows distinct cytoplasmic and extracellular positivity in renal tubules.  

Expression

 RNA: detected in 5 tissues
Protein: detected in 57 cell types		   RNA: detected in 5 tissues
Protein: detected in 54 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:15   1:600  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA041403

  

Antibody HPA041471

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 163.9   163.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041403

  

Antibody HPA041471

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA041403

  

Antibody HPA041471

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 76   61  

Antigen sequence

 
QFSINTTSISVNKLFVRVFTVHPNLCFHYSWVAEDHQGAQHTANRVFSLS
GSYIHLEPVAGTLPCGHTETITAHYT
  
VSSVYNLLTVKDLTNFPDNVDQQEEEQGHCPRPFFIHNGAIYVPLSSNEA
DIYSFLKGMGL
 

Matching transcripts

 PZP-001 - ENSP00000261336 [100%]
  PZP-001 - ENSP00000261336 [100%]
 

Other gene match

    
ANTIGEN VIEW
PZP-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.